Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Design, synthesis, biological evaluation and molecular docking study of 2,4-diarylimidazoles and 2,4-bis(benzyloxy)-5-arylpyrimidines as novel HSP90 N-terminal inhibitors

doi: 10.1080/14756366.2022.2124407

Figure Lengend Snippet: Western blotting assay. (a) (b) Effects of 16l (L: 6 μM; H: 18 μM) and 22k (L: 8 μM; H: 24 μM) on the expression of AKT, ERK, HSP70, and HSP90 in MCF-7 cells, with GAPDH as an internal reference. 17-AAG (15 μM) was used as a positive control. (c) (d) Statistical analysis of western blotting assays of 16l and 22k , respectively. Data are presented as the means ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control.

Article Snippet: Primary antibodies against ERK and AKT were purchased from Beyotime (Shanghai, China).

Techniques: Western Blot, Expressing, Positive Control, Control

Journal: Journal of Advanced Research

Article Title: Efficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53

doi: 10.1016/j.jare.2023.09.041

Figure Lengend Snippet: Antibody list.

Article Snippet: Membrane blocking was performed using 5 % skimmed milk in Tris-buffered saline for 1 h, followed by incubation with the appropriate primary antibodies against total ERK (Cat No. B7074, Assay Biotechnology, Fremont, CA, USA), phosphorylated ERK (Cat No. 9101 s, Cell Signaling Technology, Danvers, MA, USA), total AKT (Cat No. CSB-PA000855, CUSABIO, Houston, TX, USA), phosphorylated AKT (Cat No. CSB-PA008120, CUSABIO), IKKα (Cat No. 11930, Cell Signaling Technology), FGFR1 (Cat No. NBP2-33784, Novus Biologicals, Centennial, CO, USA), phosphorylated FGFR1 (Tyr653, Tyr654) (Cat No. 44-1140G, Invitrogen) polyclonal Antibody, FRS2 antibody (Cat No. 3836–100, BioVision, Waltham, MA, USA), phosphorylated FRS2-alpha (Cat No. 3864S, Cell Signaling) or ACTIN (Cat No. sc-8432, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) overnight at 4 °C.

Techniques: Staining, Plasmid Preparation